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Soybeans Today January 2000

Researchers take nematode studies to molecular level

By Fred Miller

Shouhua Wang, a post-doctoral researcher, identifies the DNA fingerprints of different races of soybean cyst nematodes.Genetic markers may provide a fast means to accurately identify races of soybean cyst nematodes and give farmers a more responsive tool for defending against these destructive microscopic worms.

"Soybean cyst nematodes are the number one problem for soybeans in the United States and, probably, the world," says Bob Riggs, University of Arkansas Division of Agriculture nematologist.

"In a dry year, non-irrigated soybeans infested with soybean cyst nematodes can suffer a 10 to 30 percent yield loss," he says. In a study funded by the Arkansas Soybean Promotion Board, Riggs and post-doctoral researcher Shouhua Wang are applying a new molecular biology technique to the identification of SCN races. The amplified fragment length polymers technique, or AFLP, is the most powerful method available for DNA fingerprinting of microorganisms.

"If we have a sufficient sample, this technique will take, at most, one week to identify cyst nematode races," Wang says. Current methods take four to five weeks.

"We have established that AFLP can be used to differentiate the races, so this is very important," Wang says. "No other fingerprinting technique has permitted us to tell as much difference."

Riggs and Wang are using identified SCN samples from across the country, as well as other countries, to find genetic indicators that can differentiate the 16 known SCN races. Riggs says there is no known method that can eradicate SCN from infested soil, including keeping susceptible plants out of a field. No high-yielding soybean variety is resistant to all SCN races, so knowing which races are present in an infested field is essential to selecting a variety that will thrive.

"In any given year, 65 to 75 percent of Arkansas' soybean acreage is infested with soybean cyst nematodes," Riggs says.

Nematodes infest the plants' root systems, distorting the tissues that transport water up to the food-producing tissues. Impaired water movement in a plant can result in yellow leaves and stunted growth.

"My advice to farmers when they find soybean cyst nematodes is to rotate their crops according to U of A Division of Agriculture recommendations," he says. "Rotating crops suppresses population growth of SCN, and one year with a non-host crop can reduce a population 75 percent."

Soybeans Today January 2000
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